Journal: Journal of visualized experiments : JoVE

Article Title: A Large Animal Model for Pulmonary Hypertension and Right Ventricular Failure: Left Pulmonary Artery Ligation and Progressive Main Pulmonary Artery Banding in Sheep

doi: 10.3791/62694

Figure Lengend Snippet: Materials

Article Snippet: Octal Bridge Amplifier , ADInstruments , FE228 , Equipment.

Techniques: Saline, Adhesive, Sterility, Ointment, Control, Blocking Assay

Journal: European journal of pain (London, England)

Article Title: Hypercapnia Reduces Perceived Heat Pain in Healthy Subjects.

doi: 10.1002/ejp.70001

Figure Lengend Snippet: FIGURE 1 | Schematic of the participant setup. Left side: In-house-built wooden frame (hypercapnibar) with four Douglas bags, two for each mix- ture (normocapnic and hypercapnic) indicated by different colours (dark green and light brown). Bags of the same mixture are connected through three-way directional manual stopcocks, which are themselves connected by tubes to a central stopcock, from which another tube delivers the mix- ture to the oronasal face mask of the participant. Right side: Participant representation with three measuring sites of autonomic recordings. Oronasal face mask with sampling tube to monitor oxygen (O2) and carbondioxide (CO2), three electrodes on the torso (distally below left and below right clavicule and distally above right hip) for electrocardiography (ECG), one pair of electrodes at the left foot (attached to the big and middle toes) for recording galvanic skin response (GSR).

Article Snippet: They included threepoint electrocardiography (ECG; range: ±100 mV; FE231 Bio Amp, ADInstruments) from the upper torso using shielded lead wires (98 cm, MLA2503, ADInstruments) and stick- on disposable electrodes (MLA1010B, ADInstrument), galvanic skin response (GSR; range: ±40μS at 1 V to 20 μS conversion; FE116 GSR Amp, ADInstruments) from the foot (contralateral to the testing side) using bipolar electrodes (3 × 2.5 cm, MLT118F, ADInstruments) at the big and middle toes and oxygen/carbon dioxide concentrations (ML206 Gas Analyzer, ADInstruments).

Techniques: Sampling

Journal: STAR Protocols

Article Title: Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time

doi: 10.1016/j.xpro.2025.103624

Figure Lengend Snippet:

Article Snippet: Bridge Amp , ADInstruments , Product Code: FE221.

Techniques: Recombinant, Targeted Gene Expression, Gene Expression, Control, Software, Sterility, Membrane

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Measuring the human ventilatory and cerebral blood flow response to CO2: a technical consideration for the end-tidal-to-arterial gas gradient.

doi: 10.1152/japplphysiol.00787.2015

Figure Lengend Snippet: Fig. 1. Bland and Altman plot for agreement between PaCO2 and PETCO2 during normoxia CO2 reactivity protocol (NX; A) and hypoxia CO2 reactivity protocol (HX; B) CO2 reactivity protocols. , 8 mmHg; , 4 mmHg; , 0 mmHg; , 4 mmHg; ▫, 8 mmHg. Dotted lines represent the 95% confidence intervals, and the continuous lines represent the mean bias.

Article Snippet: Respired gas pressures were sampled near the mouth, dried with nafion tubing and desiccant, and analyzed for PETO2 and PETCO2 (ML206, ADinstruments).

Techniques:

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Measuring the human ventilatory and cerebral blood flow response to CO2: a technical consideration for the end-tidal-to-arterial gas gradient.

doi: 10.1152/japplphysiol.00787.2015

Figure Lengend Snippet: Fig. 4. Hypercapnic ventilatory reactivity data during NX (A) and HX (B) protocols. Black bar, HCVR indexed against PETCO2; open bar, HCVR indexed against PaCO2; gray bar, HCVR indexed against *Pred-PaCO2; dark gray bar, HCVR indexed against Pred-PaCO2. Mean r2 values for NX-HCVR was 0.96 0.02 for PETCO2 HCVR, 0.94 0.00 for PaCO2 HCVR, 0.95 0.02 for *Pred-PaCO2 HCVR, and 0.96 0.02 for Pred-PaCO2 HCVR. For the HX protocol the mean r2 values for HCVR was 0.99 0.01 for PETCO2 HCVR, 0.98 0.01 for PaCO2 HCVR, 0.98 0.01 for *Pred-PaCO2 HCVR, and 0.99 0.01 for Pred-PaCO2 HCVR. *P 0.05, PaCO2 HCVR vs. PETCO2, *Pred-PaCO2, and Pred-PaCO2 HCVR. †P 0.05, vs. PETCO2 HCVR.

Article Snippet: Respired gas pressures were sampled near the mouth, dried with nafion tubing and desiccant, and analyzed for PETO2 and PETCO2 (ML206, ADinstruments).

Techniques:

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Measuring the human ventilatory and cerebral blood flow response to CO2: a technical consideration for the end-tidal-to-arterial gas gradient.

doi: 10.1152/japplphysiol.00787.2015

Figure Lengend Snippet: Fig. 5. Q˙ ICA hypocapnia and hypercapnia reactivity data during NX (A) and HX (B) protocols. Black bar, internal carotid artery (ICA) reactivity indexed against PETCO2; white bar, ICA reactivity indexed against PaCO2; gray bar, ICA reactivity indexed against *Pred-PaCO2; dark gray bar, ICA reactivity indexed against Pred-PaCO2. For the NX protocol mean r2 values for HYPO-ICA CVR was 0.93 0.03 for PETCO2 CVR, 0.93 0.03 for PaCO2 CVR, and 0.93 0.03 for Pred-PaCO2 CVR. The mean r2 values for NX HYPER-ICA CVR was 0.89 0.02 for PETCO2 CVR, 0.90 0.02 for PaCO2 CVR, and 0.89 0.02 for Pred-PaCO2 CVR. For the HX protocol the mean r2 values for HYPO-ICA CVR was 0.93 0.02 for PETCO2 CVR, 0.92 0.02 for PaCO2 CVR, and 0.93 0.02 for Pred-PaCO2 CVR. Mean r2 values for HX HYPER-ICA CVR was 0.96 0.02 for PETCO2 CVR, 0.97 0.02 for PaCO2 CVR, and 0.98 0.03 for Pred-PaCO2 CVR. *P 0.05, PaCO2 HYPER-Q˙ ICA vs PETCO2, *Pred-PaCO2, and Pred-PaCO2 HYPER-Q˙ ICA reactivity. †P 0.05, vs. PETCO2 HYPER-Q˙ ICA reactivity. ‡P 0.05, vs. Pred-PaCO2 HYPER-Q˙ ICA reactivity.

Article Snippet: Respired gas pressures were sampled near the mouth, dried with nafion tubing and desiccant, and analyzed for PETO2 and PETCO2 (ML206, ADinstruments).

Techniques:

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Measuring the human ventilatory and cerebral blood flow response to CO2: a technical consideration for the end-tidal-to-arterial gas gradient.

doi: 10.1152/japplphysiol.00787.2015

Figure Lengend Snippet: Fig. 6. MCAv hypocapnia and hypercapnia reactivity data during NX (A) and HX (B) protocols. Black bar, MCAv reactivity indexed against PETCO2; white bar, MCAv reactivity indexed against PaCO2; gray bar, MCAv reactivity indexed against *Pred-PaCO2; dark gray bar, MCAv reactivity indexed against Pred-PaCO2. For the NX trail the mean r2 values for HYPO-MCAv CVR was 0.98 0.0 for PETCO2 CVR, 0.98 0.0 for PaCO2 CVR, and 0.99 0.0 for Pred-PaCO2 CVR. Mean r2 values for NX HYPER-MCAv CVR was 0.96 0.0 for PETCO2 CVR, 0.95 0.0 for PaCO2 CVR, and 0.96 0.0 for Pred-PaCO2 CVR. For the HX trial the mean r2 values for HYPO-MCAv CVR was 0.98 0.01 for PETCO2 CVR, 0.98 0.02 for PaCO2 CVR, and 0.98 0.02 for Pred-PaCO2 CVR. Mean r2 values for HX HYPER-MCAv CVR was 0.98 0.02 for PETCO2 CVR, 0.97 0.02 for PaCO2 CVR, and 0.98 0.02 for Pred-PaCO2 CVR. *P 0.05, PaCO2 HYPER-MCAv reactivity vs. PETCO2, *Pred-PaCO2, and Pred-PaCO2 HYPER-MCAv reactivity. †P 0.05, vs. PETCO2 HYPER-MCAv reactivity. **P 0.05, PETCO2 HYPO-MCAv reactivity vs PaCO2 HYPO-MCAv reactivity.

Article Snippet: Respired gas pressures were sampled near the mouth, dried with nafion tubing and desiccant, and analyzed for PETO2 and PETCO2 (ML206, ADinstruments).

Techniques:

Journal: Journal of applied physiology (Bethesda, Md. : 1985)

Article Title: Measuring the human ventilatory and cerebral blood flow response to CO2: a technical consideration for the end-tidal-to-arterial gas gradient.

doi: 10.1152/japplphysiol.00787.2015

Figure Lengend Snippet: Fig. 7. Assessment of raw and noninvasively corrected PaCO2 and PETCO2 relationship during NX and HX CO2 reactivity protocols. Data points were obtained during the last minute of each step change in PETCO2. A: pooled linear regression for PaCO2 and PETCO2. B: pooled linear regression for PaCO2 and PETCO2 from the current study data that has been adjusted using an algorithm proposed by Tymko et al. (37) [eTymkoPaCO2 0.363 (0.958·PETCO2)]. C: pooled linear regression for PaCO2 and PETCO2 from the current study data that has been adjusted with an algorithm proposed by Peebles et al. (26) (ePeeblesPaCO2 2.367 0.884·PETCO2). b[1], slope; b[0], y-intercept. Dashed line represents the linear regression. Solid line indicates a line of identity (i.e., x y).

Article Snippet: Respired gas pressures were sampled near the mouth, dried with nafion tubing and desiccant, and analyzed for PETO2 and PETCO2 (ML206, ADinstruments).

Techniques: